[Histonet] H2DCFDA and Antifadent....
I am using H2DCFDA for the detection of cellular reactive oxygen species following treatment with various agents. Cells are subsequently mounted in anti-fadent with DAPI (Slow Fade Gold with DAPI).
The green fluorescence appears awfully weak. I have noticed this in past experiments looking at cytochrome c release with FITC detection. Could the antifadent be the culprit? Don't some antifadent products quench fluorescein right-off-the-bat? Would it be best to just do a standard aqueous mount and be extra careful with light exposure?
Many thanks for your assistance.
Glenn M. Krasinski
Senior Associate Scientist I, Biology
11535 Sorrento Valley Road, Suite 401
San Diego, CA 92121
Phone: (858) 720-8797
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