Re: [Histonet] vectashield
Buy VectaShield Hard Set instead.
Otherwise you can seal the edges with diluted permanent mounting
media. Xylene and toluene based mounting medias diluted to the consistency
of thin nail polish is not miscible with aqueous mounting medias. We avoid
nail polish containing isopropyl alcohol as the alcohol can leach under a
slide and cause fading of some fluorescent molecules i.e.
GFP. Unfortunately, we still store sealed slides flat.
At 06:55 AM 3/22/2005, you wrote:
>Hello, I am using Vectashield Mounting Media which does not seem to harden.
>Any suggestions on how to seal the slides for long term storage?
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Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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