Re: [Histonet] H&E slides for HT practical exam
Slides can be stained all together or one at a time. There is no way anyone
In fact, time in each solution can be different for each tissue stained.
What is important is that EACH slide be well stained - good crisp nuclei
with chromatin pattern, a minimum of three shades of eosin that does not
overwhelm the hematoxylin, etc..
If that takes taking each slide through the different solutions/stains at
different time intervals, repeating and tweaking the stain 20 times with
different time intervals from what you normally do, and changing to
different times for each block depending upon the tissue/length of time it
has been fixed/tissue thickness/etc. - then do it. It is more much more
important to know what to do to produce a well stained slide, than to
rigidly adhere to the same steps/times.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Mark Adam Tarango"
Sent: Tuesday, March 22, 2005 3:52 PM
Subject: [Histonet] H&E slides for HT practical exam
> Hi everyone. I have a co-worker who insists that she must stain all her
H&E and slides for her practical exam together at once. My supervisor and I
haven't ever heard this, and I'm hoping someone can give a reply as to
whether or not this is something that is required. She is conviced that if
she doesn't stain them all together (her H&E slides), someone will know and
fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd
like to her from you especially.
> Mark Tarango
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