Re: [Histonet] Frozen Spleen w/bubbles
Jo Dee,
Are you using a cryogun? That could be a cultprit. Also, are putting the slides directly into your fixative? What kind of stainer are you using? If your using a chain, put it in the heated section for 5 to 30 seconds. I used to have this problem and when we put it in the heated section for just a few seconds>30sec that solved the problem. OR you could have a bad lot of slides.
Robyn
OHSU
>>> Jo Dee Fish 03/30/05 2:30 PM >>>
Hello histonetters,
I am cryosectioning unfixed fresh frozen spleens and when they are
sectioned I can see a billion bubbles under the section. Can anyone
explain-
1. What is happening?
2. How can I avoid them?
3. Is there a fixation procedure that can help?
The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee
**********************************************************************
**********
Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease
Telephone: (415) 734-5766
Fax: (415) 355-0230
E-mail: jfish@gladstone.ucsf.edu
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>