Re: [Histonet] Frozen Spleen w/bubbles

From:"Robyn Vazquez"

Jo Dee,
Are you using a cryogun?  That could be a cultprit.  Also, are putting the slides directly into your fixative?  What kind of stainer are you using?  If your using a chain, put it in the heated section for 5 to 30 seconds.  I used to have this problem and when we put it in the heated section for just a few seconds>30sec that solved the problem.  OR you could have a bad lot of slides.


>>> Jo Dee Fish  03/30/05 2:30 PM >>>
Hello histonetters,

I am cryosectioning unfixed fresh frozen spleens and when they are 
sectioned I can see a billion bubbles under the section.  Can anyone 

1.  What is happening?
2.  How can I avoid them?
3.  Is there a fixation procedure that can help?

The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee

Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-5766
Fax: (415) 355-0230

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA   94158

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