Re: [Histonet] ERK antibody

From:"Katri Tuomala"

Hi Yan,
I have done some phospho-ERK IHC on FFPE human lung tumours. My antibody is 
from Cell Signaling Technology; rabbit monoclonal phospho-p44/42 MAPK (p44 
is Erk1 and P42 is Erk2 according to the specification sheet). If your 
antibody is from another company, my protocol may not work on yours.
I do HIER in citrate buffer after blocking endogenous peroxidases. After 
normal serum blocking, incubation with primary antibody (dilution 1:100) is 
done overnight at 4C. My secondary system is Vector Elite ABC kit. Chromogen 
Some lung tumour cells show nuclear positivity, but a variety of normal 
cells are positive also.
I hope this helps a bit...

Katri Tuomala
Hamilton, Ontario, Canada


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