Re: [Histonet] Cryosectioning for LMD POL Slides

In a message dated 3/30/2005 11:19:40 AM US Mountain Standard Time, 
kbroomal@NEMOURS.ORG writes:

> One of the other techs here is cutting snap frozen muscle for LMD and is
> having quite a time getting the sections to pick up on the POL membrane of
> the slide. She's tried warming the membrane with her finger, and I'm sure a
> couple other tricks, but barely any tissue with stick to it. Certainly not
> enough to constitute a good section.
> We're using Leica POL Membrane, metal framed slides.


A colleague of mine had exactly the same problem, with the same metal-framed 
slides.  As I recall she tried coating the membranes with polylysine, and she 
also tried treating with a silanization agent (Dow Z-6040).  I know there was 
some improvement after coating, but the process required some modifications 
because the film is so hydrophobic.  I do believe she had to add some ethanol to 
the polylysine to get the film to wet properly.  Details and concentrations 
are a little hazy...this was about a year ago.

Contact me off-list if you need additional info on the exact procedure.  I 
can put you in touch with the person who did the work.



Robert (Bob) Chiovetti, Ph.D.
Independent Consultant for The Science, Technology and Industrial Sectors
132 North Elster Drive
Tucson, AZ 85710-3212 USA
Tel./Fax 520-546-4986
Member, Arizona Small Business Association - ASBA
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