RE: [Histonet] Re:Gloves ..... a question

From:"Bartlett, Jeanine"

There was a gentleman that a friend of mine worked with for years that
always had many more problems with this than any other in the group.
They determined it was due to him excessively scratching his head near
the waterbath.  Once they brought it to his attention and he stopped, so
did the problem.  Go figure.

-----Original Message-----
[] On Behalf Of
Sent: Wednesday, March 23, 2005 10:28 AM
Subject: [Histonet] Re:Gloves ..... a question

I also have never worn gloves for embedding or cutting paraffin
material although I can see the merit of wearing gloves when cutting 
unfixed frozen material. I was also intrigued by the comments about dead

skin cells occurring on slides. It is highly unlikely that you would
identify the fragments of keratin that might be shed from your hands
cutting sections; you just would not get cornified epithelium shed from 
the hands or scalp because these sites are heavily keratinised. You are 
much more likely to get the cornified cells deposited on slides from the

mouth, by breathing on the block whilst cutting or onto the slide whilst

cleaning. I have also noticed that there are times when deposited 
cornified cells increase to problem levels and this often coincides with

the microtomist having a cold or a sore throat. I strongly feel that the

notion of cornified cells from the hand/scalp is a myth that is passed 
from generation to generation often by practitioners who should have
it more thought.

>>>>"Robyn Vazquez" 
Subject: Re: [Histonet] Gloves ... a question
I have never worn gloves and I have never had a pathologist complain of 
skin cells on my slide either.

>>> "Terry Murphy"  03/18/05 6:50 PM >>>
I once had a pathologist complain dead skin cells on  his slides when he

that I did not wear gloves when I was cutting.  Anyone else ever hear

from a pathologist?

>>> "Angela Bitting" 
>Thank you to everyone who replied to my question about wearing gloves 
>during embedding and cutting blocks. I intentionally did not mention 
>which side of the war I was on because I wanted evryone to respond 
>without feeling threatened (Histonet is such a dangerous place)LOL
>Thank you again.
>By the way,
>I'm on the side of those who DO NOT wear gloves.....

                                                    Tony Histopathology
Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines
Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire.
  SG1 2NY
tel.          +44 (0)1438 764117
fax.         +44 (0)1438 764782
mobile    +44 07753609835
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