RE: [Histonet] GMS

From:"Joe Nocito"

Probably the water,
	we have dionized tanks and sometimes we have problems from tank to tank. it
took a while to figure this out. If you can, purchase dionized water from a
vendor. I know this is costly, but if you use it just for GMS stains, you
have to figure the cost of repeats, tech time, delays in completing cases.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
[]On Behalf Of DeLovino,
Salvacion S.
Sent: Thursday, March 31, 2005 12:31 PM
To: ''
Subject: [Histonet] GMS

Is your deionized water still good? We had the same problem one time and the
culprit was the water.



>From: "Karen Harmon" 
>Subj>From: "Karen Harmon" 
>Subject: [Histonet] Pneumocystis carnii staining
>Date: Mon, 28 Mar 2005 16:25:33 -0600
>We use a GMS stain to demonstrate pneuomcyctis with successful results
>about 50% of the time.   We have taken this procedure apart step by step,
>solution by solution and are at our wits end to determine the cause for
>failure.  I can assure you that over the past 3 weeks, there is no variable

>that we have not tried.    The ironic part is we never have a problem
>demonstrating fungi with the GMS, in fact, we never had any problems at all

>with other silver procedures.
>Can anyone help with our hit and miss problem with pneumocystis?

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