RE: [Histonet] GMS

From:"Yaskovich, Ruth A (NIH/NIDCR)"

I always use Ultra Pure water for all my Silver Staining. Getting beautiful
results. Armed Forces Institute of Pathology, Neuropathology gave me this
hint.
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Crainiofacial Research
Pain and Neurosensory Mechanism Branch
Drug Discovery

> ----------
> From: 	DeLovino, Salvacion S.
> Sent: 	Thursday, March 31, 2005 12:31 PM
> To: 	'histonet@lists.utsouthwestern.edu'
> Subject: 	[Histonet] GMS
> 
> Is your deionized water still good? We had the same problem one time and
> the
> culprit was the water.
> 
> Salve
> 
> 
> 
> 
> ------------------------------
> 
> >From: "Karen Harmon" 
> >To: 
> >Subj>From: "Karen Harmon" 
> >To: 
> >Subject: [Histonet] Pneumocystis carnii staining
> >Date: Mon, 28 Mar 2005 16:25:33 -0600
> >
> >We use a GMS stain to demonstrate pneuomcyctis with successful results 
> >about 50% of the time.   We have taken this procedure apart step by step,
> 
> >solution by solution and are at our wits end to determine the cause for 
> >failure.  I can assure you that over the past 3 weeks, there is no
> variable
> 
> >that we have not tried.    The ironic part is we never have a problem  
> >demonstrating fungi with the GMS, in fact, we never had any problems at
> all
> 
> >with other silver procedures.
> >
> >Can anyone help with our hit and miss problem with pneumocystis?
> >
> ------------------------------
> 
> 
> 
> 
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