RE: [Histonet] Frozen Spleen w/bubbles

From:Kristen Broomall

I'm sure someone more knowledgeable will come along soon, but could it be
freezing artifact? How are you freezing the samples?

Kristen Broomall

-----Original Message-----
[]On Behalf Of Jo Dee
Sent: Wednesday, March 30, 2005 5:31 PM
Subject: [Histonet] Frozen Spleen w/bubbles

Hello histonetters,

I am cryosectioning unfixed fresh frozen spleens and when they are 
sectioned I can see a billion bubbles under the section.  Can anyone 

1.  What is happening?
2.  How can I avoid them?
3.  Is there a fixation procedure that can help?

The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee

Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-5766
Fax: (415) 355-0230

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA   94158

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