RE: [Histonet] Automated Microtomes

From:"Favara, Cynthia (NIH/NIAID)"


I have 2 Leica's 2255. I love them. I use them daily they have saved my
wrist and shoulder. You can use them in automatic or manual mode. It is easy
to go between manual and automatic. I have a foot pedal and I find it to be
very efficient. 

I am strictly a research facility and work primarily on rodents infected
with scrapie. We are working on numerous transgenic mice with deer/elk and
other PrP genes. I wanted a microtome that I could use in a bio-safety hood
so I could trap all the paraffin scraps when I am cutting. If you have ever
cut mouse tissue you will appreciate that even in the best of circumstances
it is very dry. I use the automatic mode on the tiniest of samples. Maybe
not quite a tiny as biopsies but mouse lymph nodes are pretty small. Some of
my work requires me to cut brain with precision so that we can sample
multiple animals in a specific anatomic region. I have never had any
difficulty doing this in the automatic mode.

Can't say enough wonderful things about this microtome!

Happy Shopping!


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: [] 
Sent: Friday, March 25, 2005 7:38 AM
Subject: [Histonet] Automated Microtomes

Are there Histotechs who actually use automated microtomes daily?   Not
the ones you have purchased, then seldom use or use in a manual mode.  I
need real-time guidance from techs who use them daily and  what
likes/dislikes you have with the particular brand bought.
Any response is appreciated.  Thanks.

Renee Grow, BA., HT (ASCP)
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax

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