[Histonet] (no subject)

From:"Margaryan, Naira"

Hello, all,


I am going to do Immunostaining of Frozen Sections .

I have some questions:

1. What to do with slides after remove them from --80 o C freezer?

2. How long the slides have to come to room temperature?

3. How the OCT compound has to be dissolved?

4. What is general protocol for frozen sections?

5. Am I have to apply Avidin/biotin?



My appreciation for any suggestion,



Naira V. Margaryan, Ph.D, D.V.M.

Research Associate II

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-880-4000/5-6740

Fax: 773-755-6594



For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314


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