[Histonet] Tissues ffpe with long fixation time
What extreme methods would you all recommend for getting at antigen sites on
tissue that has been aldehyde fixed for nearly one month? I am working with
pig heart samples, trying to do cd31, cd3, mac 387 all of which have been
worked out wonderfully on my pig tonsil control tissue aldehyde fixed for a
max of 48 hr. These are ffpe by the way. As mentioned the controls are
staining beautifully but I get nothing on the samples of interest.
Patsy
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