[Histonet] Stability of Phosphorylated Antigens in Paraffin Sections



Does anyone have information, knowledge, experience or publications on the 
stability of phosphorylated antigens / epitopes in FFPE sections?  Is it 
important to carry out any phospho-specific IHC asap after sectioning? 
 Does the phosphorylated sites decrease in antigenicity while being stored 
in sections or should they be preserved if sections are stored at 4degC as 
is the case with other (unphosphorylated) antigens?  Not sure whether this 
has been discussed before, have searched the archives, but maybe not using 
the correct search terms.

Any comments or thoughts would be greatly appreciated.

Nicola Cragg
Manchester, UK

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