[Histonet] Immunofluorescence and background
My project is based on breaking immune tolerance to the self-antigen
flk-1 for tumour therapy.
I am now trying to detect antibodies to flk-1 in whole mouse serum by
immunofluorescence. The Flk-1 receptor is expressed on transfected BHK
cells and I incubate the mouse serum at dilutions of 1:50, 1:100, and
1:200 with the cells overnight at 4 degrees celcius. There are positive
cells but the background is really obscuring the whole picture.
I would very much appreciate if anyone felt they could offer
suggestions on eliminating the background on the immunofluorescence.
Thanking you in advance.
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