Hello histonetters,

I am cryosectioning unfixed fresh frozen spleens and when they are 
sectioned I can see a billion bubbles under the section.  Can anyone 
explain-

1.  What is happening?
2.  How can I avoid them?
3.  Is there a fixation procedure that can help?

The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee
********************************************************************** 
**********

Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-5766
Fax: (415) 355-0230
E-mail: jfish@gladstone.ucsf.edu

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA   94158

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet