[Histonet] 2) Immunofluorescence and background

From:Cath Gorrie


I have a different but similar problem with Immunofluorescence and
background. I'm cutting rat spinal cord sections that have been perfused and
post fixed in 4% paraformaldehyde, cryoprotected and cut as frozen sections.


The autofluorescence in the tissue is so high I am unable to clearly see
fluorescent labelled cells.


Is this a problem of the fixation in 4% paraformaldehyde? or a property of
spinal cord? or is there something obvious I should or shouldn't be doing?


Regards, cath

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Catherine Gorrie
School of Medical Sciences
University of New South Wales
Sydney, NSW

ph: 02 9385 2462
fax: 02 9385 8016
e-mail: c.gorrie@unsw.edu.au
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