Re: [Histonet] mouse heart morphology

From:Gayle Callis

Are you freezing inside the cryostat or using a solvent/liquid nitrogen 
method, or dry ice/isopentane snap freezing?

When we have frozen mouse heart, for that matter, any murine tissues inside 
a cryostat using Peltier device cooling platforms, we get horrible freezing 
artifact with terrible morphology.  Murine tissue snap freezing tends to be 
more stringent, and unfortunately the damage caused by slower, lower 
temperture inside cryostat freezing method.

Can you use your device with liquid nitrogen surrounding it?  If so, then 
murine tissue morphology would be greatly improved.

  At 10:17 AM 3/7/2005, you wrote:
>Dear David,
>I have developed a system for embedding frozen sections that will allow 
>you to section
>your mouse hearts any way you desire. The embedding is done face down in
>  freezing temperature well bars. If you visit my web site you will see a 
> display of
>  many techniques possible with this simple apparatus. I am also just 
> introducing  with a
>larger well bar (30 x 45 mm wells )and chucks  which will allow you to 
>embed a whole mouse.
>Please feel free to call me if you have any questions.
>Stephen Peters M.D.
>Pathology Innovations, LLC
>410 Old Mill Lane,
>Wyckoff, NJ 07481
>201 847 7600
>Senior Attending Pathologist
>Hackensack University Medical Center
>201 996 4836
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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