Re: [Histonet] immunos on whole rat corneas

From:Gayle Callis


I am sending an attachment to you privately although for mouse eye IHC.  It 
was elegant and could be used for rat eyes, with some modifications for eye 
size, if needed.  The author was accessible via email also. They did no 
special permeabilization for cell surface markers but did use saponin 
permeabilization for their cytokine work for corneas on whole eye PLP fixed 
paraffin embedded sections.

J of General Virology, Stumpf et al, 83(7):1579, 2002

At 10:28 AM 3/17/2005, you wrote:
>Hello everyone
>Has anyone had any experience in immunostaining whole rat corneas?  My
>biggest concern is permeabilizing the cornea to get sufficient
>penetration.  In the past I have used 0.1% Triton X-100 for 50 micron
>free floating vibratome sections and that seemed to work quite well for
>that application.  Any suggestions would be appreciated.  Thanks in
>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>Premier Laboratory, LLC
>P.O. Box 18592
>Boulder, Colorado 80308
>Office: (303) 735-5001
>Fax: (303) 735-3540
>Ship to Address:
>Premier Laboratory
>University of Colorado
>MCDB, Room A3B40
>Boulder, Colorado 80309
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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