RT,
The reason frozen sectioning is recommended for CF is that flurescent
labels are often used and usually work best on frozens, you can rinse the
PFA from your fixed hearts with PBS or Tris buffer and then try freezing
them to make sections for IHC.
Patsy
> Hi, I have fixed some E13.5 and E16.5 mouse hearts in 4% PFA O/N and
> now i am thinking of doing confocal microscopy. What is the best way to
> embed? I see in the literature that cryo sections are better, since i
> already fixed them what would be the best bet?
> Thanks
> RT
>
>
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