Re: [Histonet] TH and golgi-cox?
Golgi-Cox is for showing dendritic structures of
individual neurons. It won't help much for recognizing
the 6 layers of the cerebral cortex. For this use
a Nissl stain, which is any cationic dye with fairly
small, flat molecules. Examples are toluidine blue,
thionine, cresyl violet and neutral red. The stain
should be differentiated until only the Nissl bodies
of neurons and the nuclei (most are of glial cells) are
coloured. If the tyrosine hydroxylase immunohistochemistry
is done with a permanent chromogen such as DAB, do the
Nissl stain afterwards, and make it light enough to
see the TH+ve fibres.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
ninian H wrote:
> Hi all,
> Does anyone know about combining golgi-cox with tyrosine hydroxylase staining? the main purpose of mine is to distinguish different layers of cortex and TH positive fibers. I'll appreciate any information that might help me to see both pyramydal cells and TH containing fibers in cortical areas.
> Also co-staining with TH and neutral red didn't work out, and TH -stained neurons didn't stain with red color , any idea or suggestion?
> Thank you in advance,
> Ninian, ph.D student
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