The Churukian Shenk toluidine blue - a wonderfully simple method never 
gives you a lot of background staining in other tissues.  I am doing to 
attach it to you privately. I know it works on paraffin sections from 
animal tissues and is NOT affected by decalcification methods either.

Plus charge slides should work perfectly.


  At 07:31 PM 3/7/2005, you wrote:
>
>Hi all,
>
>
>
>We are looking for the Mast cells in rat brain. I have free floating 
>tissue sections. The whole brain is fixed in formalin, sucrose and finally=20
>cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know=20
>what would be the most suitable protocol for staining the Mast cells with=20
>Toludine blue. I would also like to know if I could use positively charged=20
>glass slides instead of gelatin coated. Finally, I would like to know the=20
>most stable concentration for Toludine blue stock solution.
>
>Best regards,
>
>Amira
>
>**********************************************************************************************************************************
>Amira Fitieh
>Research student
>Arvid Carlsson Institute for Neuroscience
>Göteborg University
>Medicinaregata 11
>Box 432
>405 30 Göteborg
>***********************************************************************************************************************************
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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