Re: [Histonet] LCA antibody for B5 tissue
We have used Dako's M0701, clones (2B11 + PD7/26) on B5 fixed material for
lo' these many years with NO trouble.
It's even mentioned on the product data sheet.
HIER is not required! However, you should treat B5-fixed material with the
iodine/hypo sequence to remove mercury fixation pigments(even if you don't
Dilution=1:100 incubate 60 min @ RT.
----- Original Message -----
From: "Moklebust, Amanda C"
To: "'Patti Loykasek'" ; "histonet"
Sent: Wednesday, March 09, 2005 4:11 PM
Subject: RE: [Histonet] LCA antibody for B5 tissue
I have been pretreating the slides with Dako's Target Retrival
Solution pH 6.1 in a steamer for 20 minutes. The slides do not get treated
to remove mercury pigment. I've gotten two replies recommending a different
clone (2B11/PD7/26) from Dako. I don't think they were posted to everyone.
Thanks for the help.
From: Patti Loykasek [mailto:firstname.lastname@example.org]
Sent: Wednesday, March 09, 2005 12:29 PM
Subject: Re: [Histonet] LCA antibody for B5 tissue
Iım almost positive Iıve had this antibody work on B5 fixed tissues,
mainly bone marrows. What type of antigen retrieval are you using? This
could make a difference. Are you doing any type of treatment to remove the
precipitated mercuric chloride? This can lead to false negatives with some
Iıve routinely used a citrate based heat pretreatment. This recovers the
antigen nicely and removes the mercuric chloride(in most cases).
Hope this helps.
This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.
Histonet mailing list
Histonet mailing list
<< Previous Message | Next Message >>