RE: [Histonet] removal of carbohydrates/glycosylation

From:"Edmondson David (RBV) NHS Christie Tr"

I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with.  Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof.
Any suggestions/

-----Original Message-----
[]On Behalf Of Sharon
Sent: 02 March 2005 15:02
Subject: [Histonet] removal of carbohydrates/glycosylation


Does anyone know of a method to remove carbohydrate 
modifications/glycosylation from proteins in tissue sections on 
slides prior to immunohistochemistry?  (I know it's a weird 
question.)  Any suggestions, references, etc. would be greatly 

Sharon Cooperman        	     
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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