RE: [Histonet] removal of carbohydrates/glycosylation
From: | "Edmondson David (RBV) NHS Christie Tr" |
I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with. Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof.
Any suggestions/
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sharon
Cooperman
Sent: 02 March 2005 15:02
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] removal of carbohydrates/glycosylation
Hi,
Does anyone know of a method to remove carbohydrate
modifications/glycosylation from proteins in tissue sections on
slides prior to immunohistochemistry? (I know it's a weird
question.) Any suggestions, references, etc. would be greatly
appreciated.
Thanks,
Sharon
--
Sharon Cooperman
NIH, NICHD, CBMB 301.435-8417
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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