RE: [Histonet] antibody anti mouse serum amyloid A (SAA)

From:"Elizabeth Chlipala"


There is not a commercially available antibody to mouse saa.  All the
reference literature talk about a rabbit anti-mouse saa-1, but this
antibody is not available commercially; you have to get it made.  I did
try an antibody to saa from abcam it was a mouse anti-human that was
supposed to work in paraffin sections, but I could not get this to work
in mouse tissue, my negative controls stained as positive as the
antibody.  I stopped working on it after awhile.  


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Dumont,
Sent: Tuesday, March 01, 2005 12:52 PM
Subject: [Histonet] antibody anti mouse serum amyloid A (SAA)

Hi everybody!
I'm a little bit desperate.  Is there anybody here who has used a
commercial antibody against mouse serum amyloid A (SAA) for
immunohistochemistry on mouse sections?  I'm looking for a supplier but
so far, l couldn't find any.  Or if you have a protocol working on
mouse tissue with an anti-human SAA cross reacting with mouse it would
be greatly appreciated.  We tried many protocols but we've got nothing.
Please help me!!!
Nancy Dumont
Neurochem Inc.
Laval, Canada
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