Klaus Dern has disposable blade holders that fit in a regular blade holder. You might want to contact him - 706-635-8840. They set in the blade holder and tighten when you clamp it down just as you would a blade. Hope this helps.Joyce-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lett,JaclynnSent: Friday, March 11, 2005 2:52 PMTo: Gayle Callis; Histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] Reichert Histostat manualGayle,Thank you for replying.  Don Birgerson has already contacted me aboutthe manual and will send a PDF file.Here's hoping that someone on the list has one of those disposable knifeholders and no longer needs it.  Maybe we can make a deal.Thank you,Jaclynn LettSenior Research TechnicianElectron Microscopy Core FacilityDepartment of OtolaryngologyWashington University School of Medicine660 S. Euclid Ave., Campus Box 8115St. Louis, MO 63110>Voice:  747-7257Fax:  747-7230>Email:  lettj@ent.wustl.edu> -----Original Message-----From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, March 11, 2005 12:51 PMTo: Lett, Jaclynn; Histonet@lists.utsouthwestern.eduSubject: Re: [Histonet] Reichert Histostat manualJaclynn,The disposable knife holder that works in this old cryostat is the one invented for use with an AO 820 microtome many, many years ago.  It is asolid holder with a preset angle that needs no adjustment.  You can slipa high profile blade (only profile blade you can use in this holder) usinga side release lever.  The joy is holder bottom parts fit into the slotson this cryostat, and will lock securely into place.  You have to play withpicking up sections a bit but it works.There may some of these old disposable knife holders floating around storage rooms and someone willing to give one to you.Sorry, our manual is long gone but contact  Don.Birgerson@leica-microsystems.com - he is a great source of info for these old instruments.One thing, keep all sliding parts well oiled with cryostat oil in this machine is a  must but it will work.  That side arm knife holder was a horrible monster to use, and not the safest design either.At 08:24 AM 3/11/2005, you wrote:>Hello,>>We recently inherited a Reichert Histostat 855 made by Cambridge (the>manufacturer's label also says "975CJ Cryostat Microtome").  Its>operation manual could not be found.  Does anyone have a manual and>would you be willing to send us a copy?  It would be greatly>appreciated.>>Also, does anyone know of a disposable knife holder suitable for this>model?  The steel knife holder in this machine clamps the knife on its>left side.>>I hope someone can assist us.>>Thank you so much,>>Jaclynn Lett>>Senior Research Technician>Electron Microscopy Core Facility>Department of Otolaryngology>Washington University School of Medicine>660 S. Euclid Ave., Campus Box 8115>St. Louis, MO 63110>>Voice:  747-7257>Fax:  747-7230>Email:  lettj@ent.wustl.edu>>>
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