RE: [Histonet] Hematoxylin[Scanned]

From:"Jack England"

So what's wrong with being an alchemist? Those folks did a lot for the 
science of their day and whenever I spend a little time on StainsFile I 
often feel that we histo people carry on their legacy. (Sirius red F3B! 
Crystal Violet! Light Green SF Yellowish! Eye of newt! etc...)  This is one 
of the things I love about this field, that there is so much art left to the 
science.  After all, many of the most interesting discoveries and 
innovations in "modern" science of any era were and are made by curious 
folks wandering off in the weeds somewhere.

Per the hematoxylin thread, we're quite fond of Richard-Allen Scientific's 
Gill 2 hematoxylin, as well, though we've used a Weigert's formula from an 
American MasterTech kit with great results (we're a research lab, not a 
clinical one, so we run a low volume and kits are economical for us).  To 
date we've only bought solutions, but at the moment, I'm ripening a couple 
of bottles of hematoxylin right now, just to learn how it's done.

Good luck!

--Aloha,
Jack England
Tissue Genesis, Inc.
http://www.tissuegenesis.com




>From: Kemlo Rogerson 
>To: "'Bauer, Karen'" 
>CC: histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] Hematoxylin[Scanned]
>Date: Wed, 2 Mar 2005 07:49:17 -0000
>
>Good girl....
>
>Gone are the days when I had 5 litre flasks of Harris's haematoxylin
>ripening in the Stoke-on-Trent sunshine; I felt like an alchemist. Then it
>was plagiarised 'Gills' haematoxylin ripening, then some infernal lead
>haematoxylin concoction that turned everything it touched black; I 
>strangely
>put on weight too.
>
>The ripening of haematoxylin to haematin then to oxyhaematin was a delight
>to witness, the latter meant you have gone too far. The ability to carry 
>out
>an H&E progressively without having to remove the cytoplasmic 
>'overstaining'
>a delight.
>
>I'm one for keeping 'home made' haematoxylin together with retaining the 
>'a'
>in haematin, but on both points alas I feel I shall fail.
>

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