RE: [Histonet] Help

From:"Edmondson David (RBV) NHS Christie Tr"

Hi,
Did you find an answer, or ewven THE answer.  We went on to use EDTA to make sure of the immuno looking good.  Maybe the 15% Formic gave more "background" or non-specific staining.
Dave
Histology 
Christie Hosp
Manchester UK

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Traphagan, Patricia
Sent: 22 February 2005 18:05
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help


We are looking for a formic acid decalcifier for our bone marrows. We
have been told that this type of decalcifier works best on bone marrows
when a Kappa and Lambda IP stain is requested. We have tried the BBC
formic acid decalcifier, but found that the length of time it needed to
be in the decal was long (up to 4 fours) and the core was still
calcified. Are there any suggest on what could work?  Thanks

PRIVACY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain business confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If this e-mail was not intended for you, please notify the sender by reply e-mail that you received this in error.  Destroy all copies of the original message and attachments.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*******************************************************************************************************************

This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system.


*******************************************************************************************************************


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>