[Histonet] re GFP
I'll concur with john coppens .
I have done std pwax processing and still maintained good signal, provided I
aq mounted in anti fade mountant. I cut resin sections at 4microns, again
mounting with antifade mountant and was able to maintain strong enough
signal to do deconvolution/ z stacks. . Again, I concur with John's
suggestion to use an anti GFP Ab reagent. I use Molecular Probes'. Some pics
of FFPWs of fly embryos immunostained for antiGFP are in the gallery on this
site http://www.immunoportal.com/
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