[Histonet] lipids drpos inclusion in IHC for frozen sections
Hi fellows !!!
I have a prolem with IHC on frozen section ...
I can see lipid inclusion (lipids drops)... in my section when i look my immunofluorescence
I use this protocol ;
1. Formaldéhyde à 4% for 15 min
2. Bath of PBS 1x for 15 min
3. Antiboby A? against Beta-GAl(1/500) and against SCA-1(1/300)
4. 1 hour in humid chamber
5. Bath of PBS 1x for 15 min
6. 2 flurorescent secondary antibody (1/300) (Rhodamin and Fluorescein)
7. humid chamber for 30 min
8. wash with PBS
9. add mowiol for mountig scale
10. Stock at -80°C
Have you got an idea for explain my problem ... and what can i do for remove the lipids drops ? Accédez au courrier électronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34€/mn) ; tél : 08 92 68 13 50 (0,34€/mn)
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