[Histonet] Re:The best cutting angle

From:Gayle Callis

A Richard Allen MICROM microtomy specialist gave hints that Richard Allen 
Edge Rite blades high profile are approx 11 degrees, and low profile may 
need to be a bit more at 12 degrees.

However, blades from different manufacturers may have differences, although 
small and if one has difficulty with angles using any given blade, start at 
around 11 degrees or so (10 degrees would work too) and increase or 
decrease the angle in very tiny increments until you have optimal cutting 
for the blades one purchased.  Differents lots of blades from any one 
manufacturer can have differences too,  so learning to adjust angles is 
critical for good microtomy.

At 06:55 AM 3/5/2005, you wrote:

We set ours at 10, put that is personal preference.

>----- Original Message ----- From: "Hernan Aldana Marcos" 
>Subject: [Histonet] The best cutting angle
>What is the best cutting angle on the microtome for paraffins sectioning 
>using disposable blades. I have a Microm microtome that shows 0 to 17 
>variable angle in the blade holder.
>Thanks in advance

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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