[Histonet] Razor Clams


Hello Histonetters,

I have a researcher that is ageing (aging) Razor Clams and would like to
better differentiate the conchiolin, a keratin like substance, versus the
calcium carbonate of the shell.  The growth rings consist of alternating
bands of calcium carbonate and conchiolin ( as far as I can find out
conchiolin is keratin "like").  A smooth facet of the shell is produced
after a diamond saw is used to slice it in two.   The growth bands are in
the smooth facet but difficult to see.  
The bands in older animals are very close together and very difficult to
see.  Does anyone have any suggestions re: stains or procedures that might
help better differentiate the bands?  
Thanks in advance for any help.

Bill Bennett
Fisheries and Oceans Canada
Pacific Biological Station
Nanaimo, B.C. 
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