[Histonet] RE: permeabilization needed in IHC of viral protein?
Theoretically you don't need permeabilization. Since you have been
cutting sections, the cell interior is fully exposed to the
antibodies. On top of that you applied a protease step. My advise
would be testing the staining under similar conditions without and
with saponin 0.1% in all steps (including washing buffers). Again, to
my opinion a different staining pattern is not very likely.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands
----- Original Message -----
Carla M Conway
Wed, 02 Mar 2005 14:37:50 -0800
[Histonet] permeabilization needed in IHC of viral protein?
I am interested in using IHC to detect nucleocapsid (N) protein within
rhabdovirus. I have had success in detecting the glycoprotein on the
envelope surface, however the N protein is located within the virion.
Sections (FFPE) will be enzymatically digested with 0.05% protease XIV
TBS according to our standard protocol. Would a permeabilization agent
increase access to the interior proteins? Any suggestions/comments
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