[Histonet] Mast cells staining with TOL

From:"Amira Fitieh"

Hi all,


We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution.

Best regards,


Amira Fitieh
Research student
Arvid Carlsson Institute for Neuroscience
Göteborg University
Medicinaregata 11
Box 432
405 30 Göteborg
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