Good morning all!
Does anyone have experience with this antibody?  We are experiencing a
cytoplasmic "blush" in addition to very nice nuclear staining in a tumor
with a MLH1 mutation.  In order to achieve nice nuclear staining, we are
using DAKO high pH 9.9 for heat mediated antigen retrieval.  We use
EnVision+ as the detection system.   This blush may be OK so are asking
for other's experience. 
 
Thanks for any help you can give.
 
Chris Sawrenko
Histopathology
BC Cancer Agency
Vancouver, BC Canada
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