[Histonet] Cutting frozen fat

From:pam plumlee

Hi all:    I have a project that the researcher wants
frozen sections on mouse fat for immunofluorescence. 
I froze mesenteric and epididymal fat at necropsy in
isopentane.  I am having a hard time getting the
tissue to cut-I'm getting the hole in the middle of
the OCT block.  I have changed the temp in the
cryostat to range from -16 to -35 and inbetween to see
if colder would help.  Not much luck. Does anyone know
if I can defrost and fix the tissue and will it
improve the cutting or will that cause it to loose any
FITC. Thanks for any suggestions.  Pam

Do you Yahoo!? 
Yahoo! Small Business - Try our new resources site!

Histonet mailing list

<< Previous Message | Next Message >>