Hi all:    I have a project that the researcher wants
frozen sections on mouse fat for immunofluorescence. 
I froze mesenteric and epididymal fat at necropsy in
isopentane.  I am having a hard time getting the
tissue to cut-I'm getting the hole in the middle of
the OCT block.  I have changed the temp in the
cryostat to range from -16 to -35 and inbetween to see
if colder would help.  Not much luck. Does anyone know
if I can defrost and fix the tissue and will it
improve the cutting or will that cause it to loose any
FITC. Thanks for any suggestions.  Pam


		
__________________________________ 
Do you Yahoo!? 
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/ 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet