[Histonet] Calculating antibody concentrations

From:"Andrew MacDuff"

Dear All

I'm trying to find an easy way of calculating the required volumes of 2 stock antibodies to add to a fixed volume of cell suspension so that I can analyse the cells by flow cytometry at the correct Ab dilution for each Ab (ie to allow for the volume of the other Ab added to the sample so that each Ab is at its optimised titer). Can you tell me the maths needed? My poor head hurts and it has been a long time since I did anything more than use my fingers (and occasionally toes!).
Many thanks
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