Re: [Histonet] digesting soft tissue

From:Geoff McAuliffe

Household bleach, 1 part bleach to 4 parts water.
Adolph's meat tenderizer is papain, don't know the concentration needed. 
You can get papain form Sigma, works well at 37C. I don't know any other 

Karl Garsha wrote:

> Greetings,
> I'm trying to find a good cocktail for non-specific digestion of 
> tissue from bone samples--only the mineralized bone needs to be left 
> unscathed.  I'm looking for a relatively cheap solution that will 
> yield nice clean bones.  I'm aware of a product from Fisher, but it 
> costs about $70/100ml. It seems to me that there must be a simple 
> solution that I'm just not aware of (aside from culturing maggots). 
> Thanks in advance for any suggestions.
> Regards,
> Karl G.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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