Re: [Histonet] blood vessel staining?


The Pharmigen antibody works great but you may have to do some antigen 
exposure. We have found that the best staining is on thick frozen 
sections(20um) and we leave a 1:100 dilution incubating overnight AT 
Our secondary is an anti-rat biotinylated (I think this one is from 
Biomeda).  We then use the ABC kit and DAB from vector.
Good Luck

On Mar 23, 2004, at 2:46 PM, Sylvia Poulos wrote:

> Hi all,
> I've got some formalin fixed paraffin embedded mouse tissue.  Any idea
> of something I could do to stain capillaries in these?  Someone here
> mentioned mason's trichrome.  Any thoughts are always welcome. Thanks!
> Sylvia
> Sylvia P. Poulos
> USDA-ARS-Animal Physiology Research Unit
> Athens, GA 30605
> 706-583-8279
> 706-542-0399 (fax)
> _______________________________________________
> Histonet mailing list

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