Re: [Histonet] Isopropanol tissue processing
We use it routinely in our processor. We have protocols from biopsy to large
tissues. I think the times are pretty similar to xylene times, depending of
course on the tissues. If you want specifics I can email you our protocols.
Quoting "Steven E. Slap" :
> Dear HistoNetters
> For isopropanol to work as an intermedium, it has
> to be evaporated out of the paraffin. It is not,
> technically, a clearing agent. It works in
> microwave processing because the paraffin is
> heated above the boiling temperature of
> isopropanol (82°C at room temperature). I
> wouldn't think one would want to heat paraffin
> like this in a routine overnight processor.
> best regards,
> Steven Slap
> Microwave Consultant
> At 11:42 AM +1100 3/18/04, Histology wrote:
> >Hi There
> >Does anyone use Isopropanol in routine overnight
> >parraffin processing replacing xylol?? If you
> >are willing could you please supply any
> >protocols you may use.
> >Thanks for your help.
> >SDS Pathology
> >Sydney, NSW,Australia
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