Re: [Histonet] Isopropanol tissue processing

From:katherine-walters@uiowa.edu

Hi Frances,

We use it routinely in our processor.  We have protocols from biopsy to large 
tissues.  I think the times are pretty similar to xylene times, depending of 
course on the tissues.  If you want specifics I can email you our protocols.

Kathy


Quoting "Steven E. Slap" :

> Dear HistoNetters
> 
> For isopropanol to work as an intermedium, it has 
> to be evaporated out of the paraffin.  It is not, 
> technically, a clearing agent.  It works in 
> microwave processing because the paraffin is 
> heated above the boiling temperature of 
> isopropanol (82C at room temperature).  I 
> wouldn't think one would want to heat paraffin 
> like this in a routine overnight processor.
> 
> best regards,
> Steven Slap
> Microwave Consultant
> 
> At 11:42 AM +1100 3/18/04, Histology wrote:
> >
> >Hi There
> >
> >Does anyone use Isopropanol in routine overnight 
> >parraffin processing replacing xylol?? If you 
> >are willing could you please supply any 
> >protocols you may use.
> >Thanks for your help.
> >
> >Frances
> >SDS Pathology
> >Sydney, NSW,Australia
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>