Re: [Histonet] Isopropanol tissue processing
For isopropanol to work as an intermedium, it has
to be evaporated out of the paraffin. It is not,
technically, a clearing agent. It works in
microwave processing because the paraffin is
heated above the boiling temperature of
isopropanol (82°C at room temperature). I
wouldn't think one would want to heat paraffin
like this in a routine overnight processor.
At 11:42 AM +1100 3/18/04, Histology wrote:
>Does anyone use Isopropanol in routine overnight
>parraffin processing replacing xylol?? If you
>are willing could you please supply any
>protocols you may use.
>Thanks for your help.
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