Re: [Histonet] Isopropanol tissue processing

From:"Steven E. Slap"

Dear HistoNetters

For isopropanol to work as an intermedium, it has 
to be evaporated out of the paraffin.  It is not, 
technically, a clearing agent.  It works in 
microwave processing because the paraffin is 
heated above the boiling temperature of 
isopropanol (82C at room temperature).  I 
wouldn't think one would want to heat paraffin 
like this in a routine overnight processor.

best regards,
Steven Slap
Microwave Consultant

At 11:42 AM +1100 3/18/04, Histology wrote:
>
>Hi There
>
>Does anyone use Isopropanol in routine overnight 
>parraffin processing replacing xylol?? If you 
>are willing could you please supply any 
>protocols you may use.
>Thanks for your help.
>
>Frances
>SDS Pathology
>Sydney, NSW,Australia


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