Sorry, I neglected to mention that I will be working with human samples.
J

Dr. Juan Antonio Solon
MRC Keneba Field Station
MRC Laboratories Gambia
Atlantic Road, Fajara
PO Box 273, Banjul
The Gambia
Email :  juan.solon@lshtm.ac.uk
Tel No:  (++220) 541021
Fax No: (++220) 541022 / (++220) 496513
>>> Gayle Callis  03/24/04 4:41 PM >>>
What species are you working with?  Mouse?  Human?  

At 09:36 AM 3/24/2004 +0000, you wrote:
>Dear All,
>
>As part of a study on mucosal immunity in The Gambia, W. Africa, I plan
to
double stain antral and duodenal specimens for CD4 and CD25 & CD8 and
CD25.
 Also, we would attempt to triple stain for TGF beta on these subsets. 
We
have access to a Leica DMRXE although the filter cubes are all currently
long pass suppression filters . I have both formalin-fixed and frozen
sections of the antrum and the duodenum.  
>
>I would like to ask for advise on several points:
>
>1.	Can anyone share their experience on antibody combinations known
to
work for CD4-25, CD8-25 and triple staining for CD4-CD25-TGFB either
with
vectastain elite (peroxidase) or immunofluorescence.  I have compiled a
list of CD4, CD8, CD25, TGFB antibodies from Dako, Ancell, Novocastra,
BD,
and Serotec, but before purchasing any of these, I thought it would be
wise
to ask advise from the Histonet community.
>
>2.	I would probably need to acquire different filter cubes to do
simultaneous imaging of fluorescence.  There are several suppliers I
have
encountered on the net (Omega Filters and Chroma Technology) * does
anyone
have experience on using filters from these companies on Leica
microscopes
(specifically the DMRXE).  Any problems encountered with using
third-party
filters with Leica microscopes?
>
>Thanks.
>Juan
>
>
>Dr. Juan Antonio Solon
>MRC Keneba Field Station
>MRC Laboratories Gambia
>Atlantic Road, Fajara
>PO Box 273, Banjul
>The Gambia
>Email :  juan.solon@lshtm.ac.uk
>Tel No:  (++220) 541021
>Fax No: (++220) 541022 / (++220) 496513
>
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>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)




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