Re: (Fwd) Re: [Histonet] EDTA pH 8.0 recipe
I would like to point that he did NOT specify with EDTA he was using,
presumption is EDTA or EDTA disodium. Unfortunately, this is a pet peeve
of mine, one should always say WHICH EDTA is being used, or at least give a
> 1.86 gm. EDTA (same as used for decalcification)
> 5.0 liters DI water
>To the above solution, add 2 to 2.5 ml. 2N NaOH.
>Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.
>From: "Barnhart, Tammy"
>To: "Histonet (E-mail)"
>Date sent: Wed, 24 Mar 2004 07:57:44 -0600
>Subject: [Histonet] EDTA pH 8.0 recipe
>> Does anyone have a recipe to make EDTA buffer at a pH of 8.0. I would like
>> to make our own in an effort to save some dollars. I have checked the
>> archives and only have come up with one rather difficult procedure. Thanks
>> in advance....
>> Tammy Barnhart, BS, HTL(ASCP)
>> Anatomic Pathology Supervisor
>> St. Alexius Medical Center
>> Bismarck, ND
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