RE: [Histonet] smears for IHC
A point worth mentioning here is that fixation of smears can be
accomplished using this vapor method with a variety of fixatives,
formaldehyde, glutaraldehyde, acetone, ethanol, osmium tetroxide etc.
The major advantage of vapor fixation is that the tissue is not exposed
to the vehicle containing the fixative. Because of this thin layers such
as smears tend to stay on the slides better than if placing these in a
solution of the fixative. It also eliminates the need to worry about
osmolarity, concentration and pH. It is however, only useful for thin
layers, cryostat sections etc.
Barry
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Dorothy.L.Webb@HealthPartners.Com
Sent: Wednesday, March 24, 2004 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] smears for IHC
Whenever we do IHC on smears, we fix the smears in equal parts of
hydrogen peroxide and methyl alcohol for 15 minutes. Another method
that can be used is to place smears in a coplin jar and place
37%formaldehyde soaked gauze in the lid. Cover the smears for 20
minutes. Hope this helps! Dorothy Webb at Regions Hospital, St. Paul,
Minn,
-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu]
Sent: Tuesday, March 23, 2004 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 4, Issue 27
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Today's Topics:
1. Atoska Gentry/ B Plus (Joyce Cline)
2. Ventana staining Avidin Biotin Block (Featherstone, Annette)
3. Re: Ventana staining Avidin Biotin Block (Lilia Muolo)
4. RE: Ventana staining Avidin Biotin Block (GUTIERREZ, JUAN)
5. Wendy England/ iron smears (Joyce Cline)
6. IHC position in San Francisco bay area. (Morken, Tim - Labvision)
7. Small Animal atlas (Volumes 1 and 2) are back in print!
(Gayle Callis)
8. RE: Phosphotungstic Acid Hematoxylin stain without usi ng
Zenker's (Tony Henwood)
9. please remove me from your mailing list (Anna Bozzano)
10. RE: Web site for Cytology (Kemlo Rogerson)
11. subscribe (Anne Van Binsbergen)
12. Mouse Lung (Starkus, Laurie)
13. RE: help with elastic staining (Featherstone, Annette)
14. Problems with c-fos immediate early gene labelling (komo@bu.edu)
15. RE: CD98 and PLGF (Featherstone, Annette)
16. Re: Problems with c-fos immediate early gene labelling
(Geoff McAuliffe)
17. FW: Job opening - Wildlife Conservation Society (McAloose, Dee)
18. IHC on blood and cytology smears (ICC) (Jan Shivers)
19. NYSHS 2004 Annual Meeting (Luis Chiriboga)
----------------------------------------------------------------------
Message: 1
Date: Mon, 22 Mar 2004 13:15:40 -0500
From: "Joyce Cline"
Subject: [Histonet] Atoska Gentry/ B Plus
To: "Histonet"
Message-ID: <006901c41039$af661660$1d2a14ac@wchsys.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi Atoska
I get my B Plus from Market Lab, order # ML0407, Phone # 800-237-3604. I
do not know it's components, other than formaldehyde. The company that
makes it is Advanced Biomedical Reagents & Technologies.
------------------------------
Message: 2
Date: Mon, 22 Mar 2004 13:47:13 -0500
From: "Featherstone, Annette"
Subject: [Histonet] Ventana staining Avidin Biotin Block
To: "Histonet (E-mail)"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
hi
Just wondering if anyone out there using the Ventana can tell me if they
are using Avidin Biotin Block (BlockerA and BlockerB) on all their ABC
system antibodies. Our institution is only using them on a select few
and I think we should use it on all of them.Thanks for your response
Annette Featherstone HT/MLT Kaleida Health Buffalo General Hospital 100
High St Buffalo NY 14203
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------------------------------
Message: 3
Date: Mon, 22 Mar 2004 14:21:12 -0500
From: Lilia Muolo
Subject: Re: [Histonet] Ventana staining Avidin Biotin Block
To: AFeatherstone@KaleidaHealth.Org, histonet@pathology.swmed.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hi, our lab only uses the A /B blocking on the Ventana if necessary but
usually we don't need to use it. We use the Discovery here but I do
remember using the A/B blocking more often when we had the Nexes. I
would imagine that the Benchmark would be like the Discovery.
Lil Muolo
Cancer Institute of New Jersey
>>> "Featherstone, Annette" 3/22/04
1:47:13 PM >>>
hi
Just wondering if anyone out there using the Ventana can tell me if they
are using Avidin Biotin Block (BlockerA and BlockerB) on all their ABC
system
antibodies. Our institution is only using them on a select few and I
think we should use it on all of them.Thanks for your response Annette
Featherstone HT/MLT Kaleida Health Buffalo General Hospital 100 High St
Buffalo NY 14203
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------------------------------
Message: 4
Date: Mon, 22 Mar 2004 13:21:59 -0600
From: "GUTIERREZ, JUAN"
Subject: RE: [Histonet] Ventana staining Avidin Biotin Block
To: "Featherstone, Annette" ,
"Histonet (E-mail)"
Message-ID:
Content-Type: text/plain; charset="utf-8"
We use it on everything. It's a lot easier than having a different
protocol for aech type of tissue.
Juan
-----Original Message-----
From: Featherstone, Annette
[mailto:AFeatherstone@KaleidaHealth.Org]
Sent: Mon 3/22/2004 12:47 PM
To: Histonet (E-mail)
Cc:
Subject: [Histonet] Ventana staining Avidin Biotin Block
hi
Just wondering if anyone out there using the Ventana can tell me
if they are
using Avidin Biotin Block (BlockerA and BlockerB) on all their
ABC system
antibodies. Our institution is only using them on a select few
and I think
we should use it on all of them.Thanks for your response
Annette Featherstone HT/MLT
Kaleida Health
Buffalo General Hospital
100 High St
Buffalo NY 14203
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or previous e-mail messages attached to it are
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ISTSEC@KaleidaHealth.org or call (716) 859-7777.
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------------------------------
Message: 5
Date: Mon, 22 Mar 2004 15:56:30 -0500
From: "Joyce Cline"
Subject: [Histonet] Wendy England/ iron smears
To: "Histonet"
Message-ID: <001f01c41050$277dcb00$1d2a14ac@wchsys.org>
Content-Type: text/plain; charset="iso-8859-1"
I use an old fashioned way of fixing the bone marrow smears. We had
trouble with no reaction on our smears. Now we use a coplin jar with a
small piece of paper towel in the bottom, drop 10% buffered formalin to
saturate the paper towel but not to reach the blood on the slides. Leave
the slides in for 10 minutes and stain normally. We have not had any
trouble with an iron reaction since using this method. But do not leave
the slides in more than 15 minutes max.
------------------------------
Message: 6
Date: Mon, 22 Mar 2004 14:30:56 -0800
From: "Morken, Tim - Labvision"
Subject: [Histonet] IHC position in San Francisco bay area.
To: "Histology Net List Server (E-Mail)"
Message-ID:
<0556BE8AC5551E4E8AF6BB9E42509BA217741F@usca0082k08.labvision.apogent.co
m>
Content-Type: text/plain
Lab Vision, a fast-growing biotech company located in Fremont,
California, just southeast of San Francisco, has an opening for an
Immunohistochemistry Technologist in the Quality Control laboratory. The
position entails testing of manufactured lots of antibody and detection
reagents as well as prospective reagents. The applicant must have
extensive experience in immunohistochemistry, especially in development
of new antibodies and other IHC reagents. Advancement is limited only by
personal initiative. Certification by ASCP as HT or HTL and/or QIHC is
preferred. Salary is negotiable.
Lab Vision (www.labvision.com) is a world-leading manufacturer of
IHC-automation instruments and immunohistochemistry reagents for the
pathology laboratory and biological research. Lab Vision is an Apogent
company (www.apogent.com)
If interested please send resume by email to:
Tim Morken
Product Development
Lab Vision / Neomarkers
47790 Westinghouse Dr.
Fremont, CA 94539
USA
PH: 510-991-2840
FAX: 510-991-2826
email: tpmorken@labvision.com
www.labvision.com
------------------------------
Message: 7
Date: Mon, 22 Mar 2004 16:12:28 -0700
From: Gayle Callis
Subject: [Histonet] Small Animal atlas (Volumes 1 and 2) are back in
print!
To: Histonet@lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040322161228.00bcc478@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Dear All,
For those of you who work with or dissect laboratory animals and are in
need of an atlas to guide the way.
After many years of frustration and sadness when these atlases went out
of print, they are back on the market. I saw the new atlas today, and
couldn't wait to order it. A huge silent hooray!
There are two color atlases (volume 1 is for rabbit and guinea pig and
Volume 2 is rat, mouse, and Golden hamster. Pricing for Vol 1 and Vol 2
are $139 per atlas, and that is a deal.
ISBN:0702027030
Title:Colour Atlas of Anatomy of Small Laboratory Animals, Volume 2,
mouse, rat, hamster Price:$139.00
Go to Elsevier website to order, it is very user friendly, excellent.
I just gave/ordered myself an early birthday gift!
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 8
Date: Tue, 23 Mar 2004 11:22:18 +1100
From: Tony Henwood
Subject: RE: [Histonet] Phosphotungstic Acid Hematoxylin stain without
usi ng Zenker's
To: "'Boswell'" ,
Histonet@lists.utsouthwestern.edu
Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E16A@simba.kids>
Content-Type: text/plain; charset="iso-8859-1"
Dear Boswell,
The following Cherukian's modification works very well.
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
Document Procedure:
Phosphotungstic Acid Haematoxylin
Principle:
Cherukian's modification employs an eosin solution that stains the
erythrocytes red and differentiates them from the blue fibrin.
Fixation: 10% buffered formalin.
Microtomy: paraffin sections at 5um.
Controls:
Use brain sections and section of muscle. A good stain will demonstrate
the dendrites as blue where as in a bad stain they appear light grey to
salmon in colour. Nuclei, fibrin, platelets and muscle will be blue,
red cells and collagen appear red. Muscle striations should be well
defined.
Reagents:
1. Eosin: - Warning: Flammable - see MSDS
Eosin Y, water soluble (CI 145380) 0.5g
Distilled Water 10ml
80% Ethanol 190ml
Working: 10ml stock and
Before use add 50ul glacial acetic acid.
2. 1% Periodic Acid
3. PTAH solution
Haematoxylin (CI 75290) 0.5g
Phosphotungstic Acid 10g
Distilled water 500ml
Dissolve solid ingredients in separate portions of the water. Use
gentle heat for Haematoxylin. Combine solutions when cool. Add 0.088g
potassium permanganate to ripen. The stain is ready to use.
Procedure:
1. Dewax and hydrate sections to 80% alcohol.
2. Place slides in eosin for 30 seconds.
3. Wash slides in distilled water for a few seconds.
4. Place slides in 1% periodic acid for 20 minutes.
5. Wash slides in water for 3 minutes.
6. Place slides in PTAH for 30-90 minutes in 60oC oven. Check from 30
minutes on. 7. Dehydrate, clear and mount.
Results:
Dendrites, nuclei, fibrin, platelets and muscle - blue
Red blood cells and collagen - red.
Notes:
Reference:
1. Cherukian, C.J., Histologic. 8(4); 105, (1977).
2. Luna, L., Histologic. 5(2); 66, (1975).
-----Original Message-----
From: Boswell [mailto:kcboswell@grandecom.net]
Sent: Saturday, 20 March 2004 7:30 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Phosphotungstic Acid Hematoxylin stain without using
Zenker's
Does anyone have a procedure for Phosphotungstic Acid Hematoxylin stain
without using Zenker's. We have looked everywhere and are unable to
find it. Thank you. _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 9
Date: Tue, 23 Mar 2004 10:14:40 +0100
From: Anna Bozzano
Subject: [Histonet] please remove me from your mailing list
To: histonet@lists.utsouthwestern.edu
Message-ID: <3.0.1.32.20040323101440.0120fd88@cucafera.icm.csic.es>
Content-Type: text/plain; charset="us-ascii"
please remove me from your mailing list
------------------------------
Message: 10
Date: Tue, 23 Mar 2004 09:21:37 -0000
From: "Kemlo Rogerson"
Subject: RE: [Histonet] Web site for Cytology
To: "'Kathleen Boozer'" ,
Message-ID: <000001c410b8$3f376b80$48362850@KEMLOS>
Content-Type: text/plain; charset="us-ascii"
Cytopathnet.org I think. It sort of comes and goes depending on the
variety of virus it becomes infected with.
I'm always amused that it becomes regularly infected by viruses; sort of
gives you a feeling of its authenticity. I think it is negative for the
oncogenic variety but hope it has regular tests including a silicone
biopsy.
Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS
Tel: 0208 970 8414
Mob: 07830 196072
Mobile E-Mail kemlorogerson@3mail.com
FAX & Answer Phone 0871 242 8094
E-mail Accounts:
kemlo@tiscali.co.uk or
kemlo1@btinternet.com
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen
Boozer
Sent: 17 March 2004 17:13
To: histonet@pathology.swmed.edu
Subject: [Histonet] Web site for Cytology
Is there something like the Histonet available for Cytology? I have a
co-worker looking for perameters in quality control for her department
as she documents screening errors. Thanks!
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 23 Mar 2004 12:59:07 +0400
From: "Anne Van Binsbergen"
Subject: [Histonet] subscribe
To:
Message-ID:
<0C44F1AAEE47D54DA4210A60AB206F5E01F18A95@SKMCEMAIL.skmc.gov.ae>
Content-Type: text/plain; charset="iso-8859-1"
------------------------------
Message: 12
Date: Tue, 23 Mar 2004 07:56:38 -0500
From: "Starkus, Laurie"
Subject: [Histonet] Mouse Lung
To: Histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="ISO-8859-1"
I've recently started working with mouse lung and was given a protocol
for processing. The protocol calls for 0.85% NaCl for 60 minutes at 4
degrees celsius. Then a 1:1 0.85% NaCl to Absolute ethanol for 30
minutes at room temp. Then the processing looks rather normal. Since
my experience is with human tissue, I've never seen a sodium chloride
step in processing. What does it do? And, is it really necessary?
This is mouse lung infected with TB or cryptococcus.
Thanks in advance.
------------------------------
Message: 13
Date: Tue, 23 Mar 2004 08:34:14 -0500
From: "Featherstone, Annette"
Subject: RE: [Histonet] help with elastic staining
To: 'Patsy Ruegg' , Deb Stults
, histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
When doing the pentachrome stain do not over differentiate the elastic
too much in the ferric chloride, keep it kind of dark. The acid
solutions that follow continue to lighten the elastic fibers. Annette
FeatherstoneHT/MLT Kaleida Health
-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Friday, March 19, 2004 14:19
To: Deb Stults; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] help with elastic staining
I have this problem when I do a Pentachrome stain for elastic fibers,
the fibers stain fine when I first do the hematoxylin but as I continue
with the rest of the stain the fiber stain is lost, I have remedied this
by repeating the hematoxylin again at the end of the staining process to
get back the fiber staining. Try it.
Patsy
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Deb
Stults
Sent: Friday, March 19, 2004 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help with elastic staining
We ran into a problem today with an elastic stain on an artery
biopsy. We're pretty sure it is due to a reagent problem, but not sure
which one. The elastic fibers did not stain at all. We use Weigert's
iron hematoxylin solutions A and B, which are new bottles and worked
yesterday in another stain (we make up fresh before each use), the
Resorcin Fuchsin working solution expired last week, and our new bottle
(previously ordered) has not yet arrived, and the Van Gieson's solution
is brand new just opened today. Since the fibers are not black, that
makes me think that its the Weigert's, but since the Resorcin Fuchsin is
expired, we weren't sure if it could be the problem. If anyone has any
suggestions, please let me know asap. The pathologist doesn't want to
wait for new reagent to come in.
Thanks,
Deb and Karen
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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If you have received this message in error, please
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are the intended recipient, please e-mail
ISTSEC@KaleidaHealth.org or call (716) 859-7777.
------------------------------
Message: 14
Date: Tue, 23 Mar 2004 09:48:31 -0500
From: komo@bu.edu
Subject: [Histonet] Problems with c-fos immediate early gene labelling
To: histonet@lists.utsouthwestern.edu
Message-ID: <1080053311.40604e3f585a5@www.bu.edu>
Content-Type: text/plain
I'm hoping I could get some helpful advice on what might cause my c-fos
IHC labelling to work well in one lab, but not my own. I am running the
protocol on rat brains using a hydrogen peroxide/methanol step for
endogenous peroxidase blocking, a normal goat serum blocking step, a
c-fos Ab-5 primary from oncogene, a goat-anti rabbit biotinylated
secondary, a streptavidin horeradish peroxidase, and a nickle enhanced
DAB reaction.
Unfortunately, although I have all of the exact same antibodies,
chemicals,
and concentrations as the lab from which the protocol was developed, the
label is weak and not very specific when I run it at my home lab. In
fact, I
even took brain slices from the same rats that I was running at my lab
and
ran them at the lab where the protocol was developed, and found that the
c-fos label was beautiful with a low background.
Does anyone has any suggestions as to why this might be? One person
at the other lab suggested that possibly the wells that we run the
tissue in
were not being cleaned properly (we soak them in bleach while the other
lab uses just pex). Could this have any effect?
Thanks for any advice you can provide!!
Rob Komorowski
------------------------------
Message: 15
Date: Tue, 23 Mar 2004 09:50:21 -0500
From: "Featherstone, Annette"
Subject: RE: [Histonet] CD98 and PLGF
To: 'Patsy Ruegg' , "Histonet@Pathology. Swmed.
Edu"
Cc: "Ihcrg@Yahoogroups. Com"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
YES!!I am! Or was...
I used a pepsin pretreatment @37* for 15 minutes, Block with a protein
block, primary antibody 1:100 for 1hr, Goat secondary 30 min rt, Alk
phos 30 minutes rt, and whatever chromgen you use for alk phos, rt 40
min. My Plgf is from Santa Cruz. Annette Featherstone HT/MLT
-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Friday, March 19, 2004 13:58
To: Patsy Ruegg; Histonet@Pathology. Swmed. Edu
Cc: Ihcrg@Yahoogroups. Com
Subject: RE: [Histonet] CD98 and PLGF
I guess no one is using cd98 and/or Placental Growth Factor on ffpe
tissue????
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy
Ruegg
Sent: Wednesday, March 17, 2004 10:03 AM
To: Histonet@Pathology. Swmed. Edu
Cc: Ihcrg@Yahoogroups. Com
Subject: [Histonet] CD98 and PLGF
I am seeking those who may have experience with cd98 (h-300) Santa
Cruz-9160 and/or Abcam anti-PLGF ab9542 IHC on ffpe tissue??? Do these
antibodies work in ffpe tissue? Patsy
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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This email transmission and any documents, files,
or previous e-mail messages attached to it are
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If you have received this message in error, please
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sender or you are not sure as to whether you
are the intended recipient, please e-mail
ISTSEC@KaleidaHealth.org or call (716) 859-7777.
------------------------------
Message: 16
Date: Tue, 23 Mar 2004 10:24:38 -0800
From: Geoff McAuliffe
Subject: Re: [Histonet] Problems with c-fos immediate early gene
labelling
To: komo@bu.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <406080E6.2080605@umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Hi Ron:
komo@bu.edu wrote:
>I'm hoping I could get some helpful advice on what might cause my c-fos
>IHC labelling to work well in one lab, but not my own. I am running the
>protocol on rat brains using a hydrogen peroxide/methanol step for
>endogenous peroxidase blocking, a normal goat serum blocking step, a
>c-fos Ab-5 primary from oncogene, a goat-anti rabbit biotinylated
>secondary, a streptavidin horeradish peroxidase, and a nickle enhanced
>DAB reaction.
>
>Unfortunately, although I have all of the exact same antibodies,
>chemicals,
and concentrations as the lab from which the protocol was developed, the
label is weak and not very specific when I run it at my home lab. In
fact, I even took brain slices from the same rats that I was running at
my lab and ran them at the lab where the protocol was developed, and
found that the c-fos label was beautiful with a low background.
>
When you say you are using the "exact same antibodies, chemicals,
and ..... " do you mean the same bottle of reagent? You are carrying
the bottle or vial of reagent from one lab to another? If not, look at
the batch of antibody, your peroxide, your DAB, etc. If you are storing
antibody in a freezer at home it is being subjected to freeze-thaw
cycles (unless you have a very old freezer that does not defrost itself
on a timer). If your bottle of peroxide is more than a few months old it
may be in the process of turning into water, peroxide does that. I have
had DAB work one day and fail the next.
Since you have found that the brain slices stain well in the "other
lab", the problem must lie with your home lab. Since bleach reacts with
DAB I would avoid using staining dishes treated with bleach.
Geoff
>Does anyone has any suggestions as to why this might be? One person
>at the other lab suggested that possibly the wells that we run the
tissue
in
>were not being cleaned properly (we soak them in bleach while the other
>lab uses just pex). Could this have any effect?
>
>Thanks for any advice you can provide!!
>
>Rob Komorowski
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
------------------------------
Message: 17
Date: Tue, 23 Mar 2004 10:46:32 -0500
From: "McAloose, Dee"
Subject: [Histonet] FW: Job opening - Wildlife Conservation Society
To:
Message-ID: <9C26E1A5C9EE5F4B84216095CBE2E24A059DD6D3@WOLF.wcs.org>
Content-Type: text/plain; charset="iso-8859-1"
> Hello,
>
> I'd like to announce an immediate opening for a full-time
> histotechnician
in the pathology department at the Wildlife Conservation Society located
at the Bronx Zoo. We provide the diagnostic pathology services to one
of the largest zoological collections in the country. Applications are
being accepted until May 15, 2004 or until the position is filled.
Please contact Ms. Tawanda Williams (Human Resources; email:
twilliams@wcs.org) if you're interested in this exciting opportunity and
in becoming part of our team!
>
> Thanks for listening and we look forward to hearing from you.
>
> D McAloose, VMD, Dipl ACVP
> Head, Department of Pathology
> Wildlife Conservation Society
> Bronx, NY 10464
> (phone) 718-220-7105
> (fax) 718-220-7126
> dmcaloose@wcs.org
>
>
------------------------------
Message: 18
Date: Tue, 23 Mar 2004 10:21:54 -0600
From: "Jan Shivers"
Subject: [Histonet] IHC on blood and cytology smears (ICC)
To: "histonet"
Message-ID: <006301c410f2$f45d1ed0$78065486@vdl220FAC>
Content-Type: text/plain; charset="iso-8859-1"
Could someone please send me a protocol for performing IHC/ICC on smears
(I normally only do FFPE slides)?
I'd like info mostly on if the slides have to be coated/charged , smears
air-dried or fixed (and in what) for how long, and if you do any
modification of the immuno protocol, such as blocking endogenous
peroxidase later in the procedure (and with what concentration of H2O2),
and/or changing incubation times/dilutions.
I've done smears on very rare occasions, but have had trouble getting
the cells to remain on the uncoated slides that the smears are being
made on.
Thank you very much in advance.
Jan Shivers
Univ of Minnesota Vet Diag Lab
shive003@tc.umn.edu
------------------------------
Message: 19
Date: Tue, 23 Mar 2004 12:02:46 -0500
From: Luis Chiriboga
Subject: [Histonet] NYSHS 2004 Annual Meeting
To: Histonet
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
The 2004 New York State Histotechnology Society annual meeting will be
held at the Holiday Inn in beautiful Saratoga Springs, New York from
Friday April 30th through Saturday May 1st. This years meeting theme is
"Histology is no
mystery: Let New York State solve your problems". An e-mail mini program
is available upon request by replying to this message. For a program
and registration packet, please contact Judy LaDuc at 518-897-2247 or
jaladuc@capital.net We hope to see you there.
------------------------------
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End of Histonet Digest, Vol 4, Issue 27
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