if you are using a biotinylated antibody and/or detection system your
non-specific staining is probably due to endogenous biotin in liver and in
my experience even with the most aggressive ab block you cannot get rid of
the biotin in liver.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dixon,
Leslie E.
Sent: Thursday, March 18, 2004 8:59 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] active-Caspase


Hello all,
We are having issues with non-specific background staining.  I am interested
in anyone's protocol for anti-active Caspase-3 on mouse livers.  Any help
would be greatly appreciated.
Thanks,
Leslie

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