RE: [Histonet] active-Caspase

From:"Patsy Ruegg"

if you are using a biotinylated antibody and/or detection system your
non-specific staining is probably due to endogenous biotin in liver and in
my experience even with the most aggressive ab block you cannot get rid of
the biotin in liver.

-----Original Message-----
[]On Behalf Of Dixon,
Leslie E.
Sent: Thursday, March 18, 2004 8:59 AM
To: ''
Subject: [Histonet] active-Caspase

Hello all,
We are having issues with non-specific background staining.  I am interested
in anyone's protocol for anti-active Caspase-3 on mouse livers.  Any help
would be greatly appreciated.

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