RE: [Histonet] CD marker in zinc fixed mouse liver
From: firstname.lastname@example.org on behalf of Jorge Ivan Zapata Valencia
Sent: Sun 3/21/2004 6:59 AM
Subject: [Histonet] CD marker in zinc fixed mouse liver
I am new in immunohisotology and I have trying to stain for CD4,CD8 and
CD45R but i had problems to get good staining. I am using Pharmingen
protocol (rat primary antibody, biotinylated goat anti rat secondary
antibody, streptavidina-HRP). I have tried H2O2 as endogenous peroxidase
blocking solution (3% in Metanol) but eritrocytes are still brown after DAB.
I am blocking with white egg and skim mil, for endogenous biotin.
May one help me? May I have any good protocol?
Thank you very much.
JORGE IVAN ZAPATA
Universidad del Valle
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