[Histonet] Mouse Lung
I've recently started working with mouse lung and was given a protocol for
processing. The protocol calls for 0.85% NaCl for 60 minutes at 4 degrees
celsius. Then a 1:1 0.85% NaCl to Absolute ethanol for 30 minutes at room
temp. Then the processing looks rather normal. Since my experience is with
human tissue, I've never seen a sodium chloride step in processing. What
does it do? And, is it really necessary? This is mouse lung infected with
TB or cryptococcus.
Thanks in advance.
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