Dear listers,

I am trying to cut brains from fetal mice (E19) fixed in Paraformaldehyde
4% with the vibratome.

I have problems with the perfusion of the mother. The fixative does not
reach the fetuses and these even after 12 hours postfixation in the same
fixative are too soft to take the brains out. Could you give me some input?
Have you tried to cut in vibratome the whole head of the fetus? Is it
possible?

Thanks in advance

Pablo




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