Re: [Histonet] smooth muscle alpha actin antibody for mouse cells andmouse tissue?

From:Gayle Callis

With mouse on mouse IHC staining, have you tried the DAKO ARK kit?  We also
had less background using the Chemicon Mouse on Mouse kit, they will supply
you with a free sample kit, so be sure to ask for one.  Nice when a company
does that for us. 

At 10:20 AM 3/15/2004 -0500, you wrote:
>Hi everyone - I want to stain mouse tissues and smooth muscle cells in 
>culture for alpha-actin.  Apparently clone 1A4 (mouse monoclonal) is 
>quite popular, but my PI wants me to find an antibody from a different 
>species (rabbit, for example) to prevent excessive background staining.  
>Any recommendations?  And what dilutions do you use? Thank you!! - Jen

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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