PAS does a nice job of outlining smooth muscle cells in cross section, 
might be worth a try. Be advised that it stains the "cell coat" of the 
smooth muscle cell, not the membrane itself. Otherwise, immuno directed 
against a molecule unique to the cell membrane of skeletal muscle cells, 
assuming there is such a marker.

Geoff

Kowsz, Kim P wrote:

>Can someone please suggest a stain for skeletal muscle that will out-line
>each cross-sectional muscle fiber cell with a contrasting color.  The goal
>is to allow a computer imaging system to easily identify each individual
>cell.  
>
>Kim 
>kowszkp@groton.pfizer.com
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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